Journal: bioRxiv

Article Title: PLK4 inhibition as a strategy to enhance non-small cell lung cancer radiosensitivity

doi: 10.1101/2025.02.19.638860

Figure Lengend Snippet: Quantification and representative images of clonogenic survival analysis of H460 ( A ) and A549 ( B ) cells treated with CFI-400945 (10nM). Error bars represent standard error of the mean (SEM) for n=3 independent experiments analyzed by unpaired, two-tailed t-tests. Clonogenic survival analysis of H460 ( C ) or A549 ( D ) cells treated with vehicle (DMSO) or CFI-400945 (10nM) and the indicated dose of radiation. The results represent data from three independent experiments for each cell line. Data are represented as the mean ± SEM analyzed by two-way ANOVA testing. Clonogenic survival analysis of H460 ( E ) or A549 ( F ) cells treated with vehicle (DMSO) or centrinone (2.5 µM) and the indicated dose of radiation. The results represent data from three independent experiments for each cell line. Data are represented as the mean ± SEM analyzed by two-way ANOVA testing. G. Clonogenic survival analysis of MRC9 cells treated with vehicle (DMSO) or CFI-400945 (10nM) and the indicated dose of radiation. The results represent data from three independent experiments for each cell line. Data are represented as the mean ± SEM. H. Immunoblot of PLK4 expression in the indicated cell lines. Immunoblots are representative of three independent experiments.

Article Snippet: The human NSCLC cell lines H460 and A549, and human normal lung fibroblast cell line MRC9 were purchased from American Type Culture Collection (ATCC).

Techniques: Two Tailed Test, Western Blot, Expressing

Journal: bioRxiv

Article Title: Differential oxidative and pro-apoptotic response of cancer and normal cells to an anti-inflammatory agent CLEFMA

doi: 10.1101/2021.06.02.446782

Figure Lengend Snippet: CLEFMA inhibits viability and proliferation of cancer cells (H441, H226, and H1650) and not of normal fibroblasts (CCL151).

Article Snippet: Similarly, normal lung fibroblasts (CCL151 and MRC9 from ATCC) were grown in EMEM medium supplemented with 15% heat-inactivated fetal bovine serum.

Techniques:

Journal: bioRxiv

Article Title: Differential oxidative and pro-apoptotic response of cancer and normal cells to an anti-inflammatory agent CLEFMA

doi: 10.1101/2021.06.02.446782

Figure Lengend Snippet: Comparative cytotoxicity of CLEFMA and celecoxib in normal lung fibroblasts CCL151. Cell viability was determined at twice the IC 50 value of celecoxib and CLEFMA in H441 cells (50 µM and 12 µM, respectively; *p < 0.05 compared to control).

Article Snippet: Similarly, normal lung fibroblasts (CCL151 and MRC9 from ATCC) were grown in EMEM medium supplemented with 15% heat-inactivated fetal bovine serum.

Techniques: Control

Journal: bioRxiv

Article Title: Differential oxidative and pro-apoptotic response of cancer and normal cells to an anti-inflammatory agent CLEFMA

doi: 10.1101/2021.06.02.446782

Figure Lengend Snippet: CLEFMA treatment generates ROS in cancer cells, but not in normal lung fibroblasts. (A) Effect of CLEFMA on induction of ROS in H441 lung cancer cells and normal lung fibroblasts CCL151 in presence or absence of antioxidants (*p <0.05 compared with control). (B) CLEFMA did not induce ROS in normal lung fibroblasts CCL151 at all concentrations of CLEFMA tested. ROS was measured in terms of relative fluorescence unit (% RFU) as compared to control cells which were not treated with CLEFMA.

Article Snippet: Similarly, normal lung fibroblasts (CCL151 and MRC9 from ATCC) were grown in EMEM medium supplemented with 15% heat-inactivated fetal bovine serum.

Techniques: Control, Fluorescence

Journal: bioRxiv

Article Title: Differential oxidative and pro-apoptotic response of cancer and normal cells to an anti-inflammatory agent CLEFMA

doi: 10.1101/2021.06.02.446782

Figure Lengend Snippet: Fluorescence micrographs of cancer cells (H441, H226, and H1650) and normal fibroblasts (CCL151 and MRC9) showing differential ROS activity. The cells were stained with Image-iT LIVE Green Reactive Oxygen Species Detection kit. Blue represent nuclear staining and green punctuates track the ROS generated. Control cells received vehicle DMSO treatment whereas treated cells received CLEFMA at 10 μM concentration.

Article Snippet: Similarly, normal lung fibroblasts (CCL151 and MRC9 from ATCC) were grown in EMEM medium supplemented with 15% heat-inactivated fetal bovine serum.

Techniques: Fluorescence, Activity Assay, Staining, Generated, Control, Concentration Assay

Journal: bioRxiv

Article Title: Differential oxidative and pro-apoptotic response of cancer and normal cells to an anti-inflammatory agent CLEFMA

doi: 10.1101/2021.06.02.446782

Figure Lengend Snippet: CLEFMA induces apoptosis selectively in cancer cells. (A) Effect of CLEFMA on anti-apoptotic proteins in cancer cells (H441, H226, and H1650) and normal fibroblasts (CCL151 and MRC9). (B) Effect of CLEFMA on phospho-p53 expression and cleavage of apoptotic markers caspase 3 and PARP in cancer cells (H441, H226, and H1650) and normal fibroblasts (CCL151 and MRC9). The protein extracts were analyzed by Western blotting.

Article Snippet: Similarly, normal lung fibroblasts (CCL151 and MRC9 from ATCC) were grown in EMEM medium supplemented with 15% heat-inactivated fetal bovine serum.

Techniques: Expressing, Western Blot

Journal: bioRxiv

Article Title: Differential oxidative and pro-apoptotic response of cancer and normal cells to an anti-inflammatory agent CLEFMA

doi: 10.1101/2021.06.02.446782

Figure Lengend Snippet: CLEFMA suppresses nuclear expression and activity of NF-κB in cancer cells, but not in normal fibroblasts. A representative blot showing that CLEFMA inhibited the nuclear translocation of NF-κB phospho-p65 in all cancer cells (H441, H1650, and H226), but had no such effect in normal fibroblasts (MRC9 and CCL151).

Article Snippet: Similarly, normal lung fibroblasts (CCL151 and MRC9 from ATCC) were grown in EMEM medium supplemented with 15% heat-inactivated fetal bovine serum.

Techniques: Expressing, Activity Assay, Translocation Assay